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美洲大蠊中蛋白质和糖胺聚糖双指标的提取工艺研究
  

美洲大蠊中蛋白质和糖胺聚糖双指标的提取工艺研究

李佳怿1,米士丽2,张瑞1,陶然1,毛近隆1*

1.山东中医药大学药学院,山东济南 2503552.南京中医药大学药学院,江苏南京 2100003.山东中医药大学实验中心,山东济南 250355

[摘要] 目的:研究美洲大蠊中总蛋白质和糖胺聚糖的含量及提取工艺。方法:美洲大蠊通过水提酶解法提取,设计正交实验从料液比、超声时间和提取温度三方面考察,总蛋白质通过改良lowry法测定,糖胺聚糖通过阿利新蓝染色法测定,以蛋白质和糖胺聚糖的提取率为指标,筛选最佳提取工艺。结果:美洲大蠊中蛋白质及糖胺聚糖的最佳提取条件为:料液比1:30、超声时间70 min及温度40 ℃,其中总蛋白的提取率为27.79 %,糖胺聚糖的提取率2.31 %结论:通过美洲大蠊的双指标优化提取工艺,有利于发挥多成分功效,提高其药 1e6b 硌芯啃Ч

关键词:美洲大蠊;总蛋白质;糖胺聚糖;正交实验

Study on Extraction Technology of protein and glycosaminoglycan from Periplaneta Americana

LI jia-yi1,MI shi-li2,ZHANG rui1,TAO ran1, MAO jin-long*1

(1. College of pharmacy, Shandong University of TCM, Jinan 250355, China; 2. College of pharmacy, Nanning University of TCM, Nanjing 2100003, China; 1*.The experiment center of Shandong University of TCM, Jinan, 250355, China)

[abstract] Objective: To study the content and extraction technology of total protein and glycosaminoglycan in Periplaneta americana.Method: Periplaneta Americana was extracted by enzymatic hydrolysis with water extraction. Orthogonal experiments were designed to investigate the ratio of material to liquid, ultrasonic time and extraction temperature. Total protein was determined by modified Lowry method, and glycosaminoglycan was determined by alisine blue staining. The extraction rate of protein and glycosaminoglycan was used as index to screen the best extraction process. Result: The optimum extraction conditions of protein and glycosaminoglycan from Periplaneta Americana were as follows: ratio of material to liquid 1:30, ultrasonic time 70 min and temperature 40 C. The extraction rate of total protein and glycosaminoglycan was 27.79% and 2.31%. Conclusion: To optimize the extraction process of Periplaneta Americana with two indicators is conducive to giving full play to the efficacy of multi-components and improving the pharmacological research effect of Periplaneta Americana.
[Keywords]Periplaneta americana; total protein; glycosaminoglycan; orthogonal experiment



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